SELEX libraries typically comprise up to 100 nucleotides in length, containing a random region flanked by two constant primer-binding sites, which are used for PCR amplification of the library. After several rounds of selection and amplification the library is sequenced and monoclonal aptamer sequences are identified. The best binding sequences are recovered, amplified by PCR and the enriched library is employed in the next selection cycle. In a typical SELEX experiment a library of up to 10 15 different nucleic acid strands is exposed to a selection pressure were the best target binding sequences are partitioned from the rest. Īptamers are usually generated by an iterative in vitro selection process called “systematic evolution of ligands by exponential enrichment” (SELEX). as sensors – or as tools in chemical biology –. as therapeutics, for drug discovery, as delivery and targeting units, , diagnostics, e.g. Therefore, aptamers can be conducive to a myriad of applications in various fields such as pharmaceutical sciences, e.g. They are selected against a broad range of targets including small molecules and proteins –. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist.Īptamers are short single stranded oligonucleotides with the ability to bind their target with high affinity and high specificity. acknowledge support from the European Research Concil under the European Union's Seventh Framework program (FP7/2007–2013)/ERC Grant agreement no. All relevant data are within the paper and its Supporting Information files.įunding: Funded by Deutsche Forschungsgemeinschaft to G.M. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: The authors confirm that all data underlying the findings are fully available without restriction. Received: AugAccepted: NovemPublished: December 9, 2014Ĭopyright: © 2014 Tolle et al. PLoS ONE 9(12):Įditor: Mark Isalan, Imperial College London, United Kingdom Citation: Tolle F, Wilke J, Wengel J, Mayer G (2014) By-Product Formation in Repetitive PCR Amplification of DNA Libraries during SELEX.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |